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2018-06-04 12:15:492025-05-13 23:39:33

description

ATP-dependent DNA helicase, facilitates unwinding of ICEBs1 DNA for horizontal transfer

ATP-dependent DNA helicase, coupling of DNA replication, transcription, recombination and segregation, facilitates unwinding of ICEBs1 DNA for horizontal transfer

locus

BSU06610

BSU_06610

geneLength

2217

2220

function

plasmid rolling-circle replication, conjugative transfer of ICEBs1

coupling of DNA replication, transcription, recombination and segregation

outlinks

bsu

BSU06610

BSU_06610

Gene

Phenotypes of a mutant

essential [Pubmed|12682299]

essential [Pubmed|12682299]

inactivation can be suppressed by inactivation of either [[gene|recA]], [[gene|recJ]], [[gene|recO]], or [[gene|mfd]] [pubmed|32793628]

The protein

Catalyzed reaction/ biological activity

PcrA anchors itself to the template duplex using the 2B subdomain and reels in the lagging strand, extruding a single-stranded loop [Pubmed|20723756]

dismantles [[protein|RecA]] filaments [Pubmed|20723756]

coupling of DNA replication, transcription, recombination and segregation [pubmed|32793628]

PcrA anchors itself to the template duplex using the 2B subdomain and reels in the lagging strand, extruding a single-stranded loop [Pubmed|20723756]

dismantles [[protein|RecA]] filaments [Pubmed|20723756]

ATP + H2O --> ADP + H+ + phosphate (according to UniProt)

The protein

Protein family

uvrD-like helicase C-terminal domain (according to Swiss-Prot)

[SW|helicase family] (according to UniProt)

The protein

[SW|Domains]

two RecA-like domains [Pubmed|24147116]

intrinxically disordered C-terminal domain (for interaction with [SW|RNA polymerase]) [Pubmed|24147116]

two RecA-like domains [Pubmed|24147116]

intrinxically disordered C-terminal domain (for interaction with [SW|RNA polymerase]) [Pubmed|24147116]

[SW|UvrD-like helicase ATP-binding domain](aa 10-289) (according to UniProt)

[SW|UvrD-like helicase C-terminal] (aa 290-570) (according to UniProt)

References

Reviews

19943906

19943906, 29856930

References

Original Publications

12073041, 9701819, 16267290, 19943900, 20723756, 18276648, 17449621, 10899133, 10199404, 23326247, 24147116, 21710567, 26070154, 28160601

12073041, 9701819, 16267290, 19943900, 20723756, 18276648, 17449621, 10899133, 10199404, 23326247, 24147116, 21710567, 26070154, 28160601, 32793628